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ELISA Method: Accurate and Flexible Analysis in 96 Well Plates

DMPK (Drug metabolism and Pharmacokinetics) is a scientific discipline within drug discovery, dealing with the safety and efficacy evaluation of drug candidates before entering clinical trials. DMPK studies come along with absorption, distribution, metabolism, excretion and toxicity analysis (ADMET) of drug candidates. The DMPK discipline is the basis for optimizing compounds so that bioavailability, drug-drug interactions (DDI), and related risks to a drug compound can be evaluated. Assessment of DMPK profiles minimizes the rate of attrition of drug candidates and increases the efficiency of drug discovery overall. 

Drug metabolism and pharmacokinetics, or DMPK, is a scientific discipline within drug discovery, dealing with safety and efficacy evaluation of drug candidates before entering clinical trials. DMPK studies come along with absorption, distribution, metabolism, excretion and toxicity analysis (ADMET) of drug candidates. The DMPK discipline is the basis for optimizing compounds so that bioavailability, drug-drug interactions (DDI), and related risks to a drug compound can be evaluated.  The Drug Metabolism and Pharmacokinetics (DMPK) discipline is the basis for optimization of compounds so that bioavailability, drug-drug interactions (DDI), and related risks to a drug candidate can be evaluated. The objective of DMPK studies is to evaluate multiple properties of drugs, such as clearance, distribution volume, half-life, bioavailability, drug-drug interaction, and metabolic profile.

Enzyme Linked Immunosorbent Assay (ELISA) method is a widely utilized immunoassay for quantitating and detecting proteins, hormones, peptides and many other drugs along with their metabolites. ELISA assay are effective in quantitatively detecting any molecule or antigen that can be ascertained by an antibody. For example, ELISA methods are used in pregnancy testing, infectious disease identification and detection of cytokines and soluble receptor proteins etc. Due to the precision, sensitivity, assay speed and ease of quantitation, ELISA method development has become a common choice for several diagnostic and research applications. ELISA method is a widely utilized immunoassay for quantitating and detecting proteins, hormones, peptides, cytokines, antibodies, and other drugs along with their metabolites. ELISA methods are effective in quantitatively detecting any molecule, or antigen, that can be ascertained by an antibody. For example, ELISA methods are used in pregnancy testing, infectious disease identification, and detection of cytokines, and soluble receptor proteins, etc.

A sandwich ELISA method is the most popular assay format. In a sandwich ELISA method, your analyte is sandwiched in-between two antibodies that bind to different regions (epitopes) on your antigen. The fused antibody-antigen complex then binds to an enzyme (enzyme conjugate) that you’ve added as an additional specific binding molecule. To generate a signal, your enzyme-conjugate also must be able to react with an additional secreted substrate that causes an adaptive color change within the indicator.

ELISA may be used for the detection of hormone, cytokine or growth factor in body fluids. ELISA is a simple, fast and inexpensive method for the determination of low concentrations of biologically active substances such as cytokines, growth factors and secreted proteins. This assay method is common for several diagnostic and research applications. Due to the precision, sensitivity, assay speed, and ease of quantitation, this method development is a common choice for several diagnostic and research applications.

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